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breast epithelial cell lines mcf10a  (ATCC)


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    ATCC breast epithelial cell lines mcf10a
    Breast Epithelial Cell Lines Mcf10a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epithelial+mcf10a/MCF+10A/pm42210419-64-10-18
    Average 99 stars, based on 8098 article reviews
    breast epithelial cell lines mcf10a - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Evaluation of multidrug resistance in the Gram-negative microbiome of cancer patients and the adverse effects of their metabolites on albino rats and epithelial or fibroblasts cell lines.
    Article Snippet: .. Preparation of cell lines and The MTT cell proliferation assay and IC50 determination To study the cytotoxic effects of these microbial extracts, their anti-proliferation activities against three different types of human non-tumorigenic cells including mammary epithelial (MCF10A), lung fibroblasts (WI38) and dermal fibroblasts (HDFs) cell lines (American Type Culture Collection; ATCC) were evaluated. .. MCF10A were preserved in MEBM basal medium added with the MEGM SingleQuot Kit (Lonza Corporation, USA) containing 100 ng/mL cholera toxin (Sigma-Aldrich), WI38 were cultured in Eagle’s minimum essential medium ((EMEM, ATCC) complemented with 2 mM glutamine and 1% non-essential amino acids (NEAA) and HDFs were cultured in Dulbecco’s modified Eagle’s medium (DMEM).

    Proliferation Assay:

    Article Title: Evaluation of multidrug resistance in the Gram-negative microbiome of cancer patients and the adverse effects of their metabolites on albino rats and epithelial or fibroblasts cell lines.
    Article Snippet: .. Preparation of cell lines and The MTT cell proliferation assay and IC50 determination To study the cytotoxic effects of these microbial extracts, their anti-proliferation activities against three different types of human non-tumorigenic cells including mammary epithelial (MCF10A), lung fibroblasts (WI38) and dermal fibroblasts (HDFs) cell lines (American Type Culture Collection; ATCC) were evaluated. .. MCF10A were preserved in MEBM basal medium added with the MEGM SingleQuot Kit (Lonza Corporation, USA) containing 100 ng/mL cholera toxin (Sigma-Aldrich), WI38 were cultured in Eagle’s minimum essential medium ((EMEM, ATCC) complemented with 2 mM glutamine and 1% non-essential amino acids (NEAA) and HDFs were cultured in Dulbecco’s modified Eagle’s medium (DMEM).

    Multiple Displacement Amplification:

    Article Title: Human breast cancer-derived soluble factors facilitate CCL19-induced chemotaxis of human dendritic cells
    Article Snippet: CD14 + monocytes were cultured in RPMI 1640 medium with 5% FBS, 1% penicillin/streptomycin, 1000 units/ml GM-CSF (Invitrogen), and 1000 units/ml IL-4 (Invitrogen) for 72 h, and then the same concentrations of GM-CSF and IL-4 were added for an additional 48 h. .. Epithelial MCF10A, luminal MCF7, metastatic triple negative breast cancer MDA-MB-231 or MDA-MB-436, and HER2-overexpressing SK-BR-3 cells were purchased from the American Type Culture Collection. ..



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    Mucin 1 (MUC1) expression in human breast cancer. A , MUC1 expression levels in breast cancer (UALCAN website). B , MUC1 expression levels in different subtypes of breast cancer (UALCAN website). C , MUC1 protein expression in breast cancer (Human Protein Atlas database). D , MUC1 expression in human normal mammary epithelial cells <t>MCF10A,</t> TNBC cells (MDA-MB-231), and TNBC tissues (scale bar=100 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BRCA: breast cancer gene; TCGA: The Cancer Genome Atlas; TNBC: triple-negative breast cancer.
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    PCMT1 is involved in PTX resistance of BC cells, a process potentially involving COX-2-mediated AA metabolism. (A) RT-qPCR analysis of PCMT1 mRNA levels in tumor tissues and paired adjacent normal tissues from 30 BC patients treated with PTX. (B) Immunoblotting analysis of PCMT1 and COX-2 protein levels in tumor tissues and paired adjacent normal tissues from PTX-treated BC patients (n = 10; 5 PTX-sensitive and 5 PTX-resistant). (C) Correlation between PCMT1 and COX-2 expression was assessed using the Pearson correlation coefficient. (D) RT-qPCR analysis of PCMT1 mRNA levels in <t>MCF10A,</t> BC cells (MDA-MB-231, MCF-7), and PTX-resistant cells (MDA-MB-231/PTX, MCF-7/PTX) (n = 3). (E) Immunoblotting analysis of PCMT1 and COX-2 protein levels in BC cells and PTX-resistant cells (n = 3). (F) IC50 values of BC cells and PTX-resistant cells assessed using the CCK-8 assay (n = 3). Values are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.
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    PCMT1 is involved in PTX resistance of BC cells, a process potentially involving COX-2-mediated AA metabolism. (A) RT-qPCR analysis of PCMT1 mRNA levels in tumor tissues and paired adjacent normal tissues from 30 BC patients treated with PTX. (B) Immunoblotting analysis of PCMT1 and COX-2 protein levels in tumor tissues and paired adjacent normal tissues from PTX-treated BC patients (n = 10; 5 PTX-sensitive and 5 PTX-resistant). (C) Correlation between PCMT1 and COX-2 expression was assessed using the Pearson correlation coefficient. (D) RT-qPCR analysis of PCMT1 mRNA levels in <t>MCF10A,</t> BC cells (MDA-MB-231, MCF-7), and PTX-resistant cells (MDA-MB-231/PTX, MCF-7/PTX) (n = 3). (E) Immunoblotting analysis of PCMT1 and COX-2 protein levels in BC cells and PTX-resistant cells (n = 3). (F) IC50 values of BC cells and PTX-resistant cells assessed using the CCK-8 assay (n = 3). Values are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.
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    (A) Dose–response curves of Ga3 and Ga6 compared to cisplatin in nontransformed cell lines HaCaT and <t>MCF10A.</t> (B) Selectivity Index values for Ga3 , Ga6 and cisplatin. Data represent the mean ± SD from three independent experiments performed in triplicate.
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    Mucin 1 (MUC1) expression in human breast cancer. A , MUC1 expression levels in breast cancer (UALCAN website). B , MUC1 expression levels in different subtypes of breast cancer (UALCAN website). C , MUC1 protein expression in breast cancer (Human Protein Atlas database). D , MUC1 expression in human normal mammary epithelial cells MCF10A, TNBC cells (MDA-MB-231), and TNBC tissues (scale bar=100 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BRCA: breast cancer gene; TCGA: The Cancer Genome Atlas; TNBC: triple-negative breast cancer.

    Journal: Brazilian Journal of Medical and Biological Research

    Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

    doi: 10.1590/1414-431X2026e15075

    Figure Lengend Snippet: Mucin 1 (MUC1) expression in human breast cancer. A , MUC1 expression levels in breast cancer (UALCAN website). B , MUC1 expression levels in different subtypes of breast cancer (UALCAN website). C , MUC1 protein expression in breast cancer (Human Protein Atlas database). D , MUC1 expression in human normal mammary epithelial cells MCF10A, TNBC cells (MDA-MB-231), and TNBC tissues (scale bar=100 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BRCA: breast cancer gene; TCGA: The Cancer Genome Atlas; TNBC: triple-negative breast cancer.

    Article Snippet: The human triple-negative breast cancer cell line MDA-MB-231 and the normal human breast epithelial cells MCF10A were obtained from the Procell Biotechnology Company (China).

    Techniques: Expressing, Two Tailed Test

    PCMT1 is involved in PTX resistance of BC cells, a process potentially involving COX-2-mediated AA metabolism. (A) RT-qPCR analysis of PCMT1 mRNA levels in tumor tissues and paired adjacent normal tissues from 30 BC patients treated with PTX. (B) Immunoblotting analysis of PCMT1 and COX-2 protein levels in tumor tissues and paired adjacent normal tissues from PTX-treated BC patients (n = 10; 5 PTX-sensitive and 5 PTX-resistant). (C) Correlation between PCMT1 and COX-2 expression was assessed using the Pearson correlation coefficient. (D) RT-qPCR analysis of PCMT1 mRNA levels in MCF10A, BC cells (MDA-MB-231, MCF-7), and PTX-resistant cells (MDA-MB-231/PTX, MCF-7/PTX) (n = 3). (E) Immunoblotting analysis of PCMT1 and COX-2 protein levels in BC cells and PTX-resistant cells (n = 3). (F) IC50 values of BC cells and PTX-resistant cells assessed using the CCK-8 assay (n = 3). Values are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: LITAF suppresses breast cancer and paclitaxel resistance by ubiquitinating and degrading PCMT1 to inhibit COX-2-dependent arachidonic acid metabolism

    doi: 10.3389/fphar.2026.1706420

    Figure Lengend Snippet: PCMT1 is involved in PTX resistance of BC cells, a process potentially involving COX-2-mediated AA metabolism. (A) RT-qPCR analysis of PCMT1 mRNA levels in tumor tissues and paired adjacent normal tissues from 30 BC patients treated with PTX. (B) Immunoblotting analysis of PCMT1 and COX-2 protein levels in tumor tissues and paired adjacent normal tissues from PTX-treated BC patients (n = 10; 5 PTX-sensitive and 5 PTX-resistant). (C) Correlation between PCMT1 and COX-2 expression was assessed using the Pearson correlation coefficient. (D) RT-qPCR analysis of PCMT1 mRNA levels in MCF10A, BC cells (MDA-MB-231, MCF-7), and PTX-resistant cells (MDA-MB-231/PTX, MCF-7/PTX) (n = 3). (E) Immunoblotting analysis of PCMT1 and COX-2 protein levels in BC cells and PTX-resistant cells (n = 3). (F) IC50 values of BC cells and PTX-resistant cells assessed using the CCK-8 assay (n = 3). Values are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Human normal mammary epithelial cells MCF10A (ATCC, USA) were cultured in DMEM/F12 (Procell) supplemented with HS (5%), EGF (20 ng/mL), hydrocortisone (0.5 μg/mL), insulin (0.5 μg/mL), NEAA (1%), and P/S (1%).

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, CCK-8 Assay

    (A) Dose–response curves of Ga3 and Ga6 compared to cisplatin in nontransformed cell lines HaCaT and MCF10A. (B) Selectivity Index values for Ga3 , Ga6 and cisplatin. Data represent the mean ± SD from three independent experiments performed in triplicate.

    Journal: Journal of Medicinal Chemistry

    Article Title: Exploring Gallium(III) Complexes as Emerging Therapeutic Candidates for Breast Cancer

    doi: 10.1021/acs.jmedchem.5c03480

    Figure Lengend Snippet: (A) Dose–response curves of Ga3 and Ga6 compared to cisplatin in nontransformed cell lines HaCaT and MCF10A. (B) Selectivity Index values for Ga3 , Ga6 and cisplatin. Data represent the mean ± SD from three independent experiments performed in triplicate.

    Article Snippet: The cell lines MCF7, SKBR3, BT549, and MDA-MB-231, the immortalized nontransformed keratinocyte cell line HaCaT, and the human mammary epithelial cell line MCF10A were acquired from ATCC.

    Techniques:

    Matrigel assay. (A) 3D structures of MCF7, MDA-MB-231 and MCF10A seeded in Matrigel matrix and treated with compound at indicated doses for 72-h. Scale bar = 250 μm. (B) The diameter of 3D structures was quantified using ImageJ. Data are the average ± SEM of three independent experiments. The values for the statistical analyses are * p ≤ 0.05; *** p ≤ 0.001.

    Journal: Journal of Medicinal Chemistry

    Article Title: Exploring Gallium(III) Complexes as Emerging Therapeutic Candidates for Breast Cancer

    doi: 10.1021/acs.jmedchem.5c03480

    Figure Lengend Snippet: Matrigel assay. (A) 3D structures of MCF7, MDA-MB-231 and MCF10A seeded in Matrigel matrix and treated with compound at indicated doses for 72-h. Scale bar = 250 μm. (B) The diameter of 3D structures was quantified using ImageJ. Data are the average ± SEM of three independent experiments. The values for the statistical analyses are * p ≤ 0.05; *** p ≤ 0.001.

    Article Snippet: The cell lines MCF7, SKBR3, BT549, and MDA-MB-231, the immortalized nontransformed keratinocyte cell line HaCaT, and the human mammary epithelial cell line MCF10A were acquired from ATCC.

    Techniques: Matrigel Assay